|
KMID : 0545119960060030184
|
|
Journal of Microbiology and Biotechnology
1996 Volume.6 No. 3 p.184 ~ p.188
|
|
|
Purification and Characterization of Bioemulsifier Produced by Acinetobacter sp. BE-254
|
|
Kim, Soon Han
Lee, Jae Dong/Kim, Boo Chul/Lee, Tae Ho
|
|
|
Abstract
|
|
|
|
The Acinetobacter sp. BE-254 isolated from soil sources produced a bioemulsifier in the medium supplemented with n-hexadecane. This bioemulsifier was purified by the procedures of fractionation (ammonium sulfate and chilled acetone), extraction by hexane, and column chromatography on silica gel 60. The results from various color reactions indicated that the bioemulsifier was a glycolipid. The purified emulsifier was very stable at pHs ranging from 4 to 10 and under heat treatment at 100℃ for 30 min. Emulsification activity was also hardly influenced by pH. The critical micelle concentration (CMC) and surface tension at the point (γ_cmc) of the bioemulsifier were approximately 35 ㎎/ℓ and 30 mN/m, respectively. The bioemulsifier showed a fairly good emulsification activity and stability in comparison with other commercial emulsifiers in the basic formula composed of emulsifier, oil, and water.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|